Coding

Part:BBa_K2888001:Design

Designed by: SBS_SH_112144   Group: iGEM18_SBS_SH_112144   (2018-10-06)


mlrA gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 709
    Illegal AgeI site found at 244
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We copied the sequence from the experiment by Kazuya Shimizu. However, we replaced unexpected restriction site with corresponding code


Source

It is from the microcystin degradation system discovered in Sphingopyxis sp. C-1(Okano et al. 2009)

References